CYTOGENETICS OF FRAGMENT CHROMOSOMES IN BARLEY

Avian Meiotic Chromosomes as Model Objects in Cytogenetics

Sponge cytogenetics - mitotic chromosomes of ten species of freshwater sponge.

Porifera (sponges) are the most basal phylum of extant metazoans. To gain insight into sponge genome construction, cytogenetic analysis was performed for ten freshwater sponge species of six genera, using conventional Giemsa staining, chromosome banding, and fluorescence in-situ hybridization. The karyotypes were very similar among the ten species, exhibiting a diploid chromosome number of 2n=4...

Impact of Mutagens on DNA Replication in Barley Chromosomes.

Replication errors that are caused by mutagens are critical for living cells. The aim of the study was to analyze the distribution of a DNA replication pattern on chromosomes of the H. vulgare 'Start' variety using pulse 5-ethynyl-2'-deoxyuridine (EdU) labeling, as well as its relationship to the DNA damage that is induced by mutagenic treatment with maleic hydrazide (MH) and γ ray. To the best...

Characterization of the late replication banding patterns in barley chromosomes

Replicating DNA regions on barley chromosomes were investigated by a replication banding technique. Of all chromosomal regions, the late replicating regions, in which DNA replicated in the last third of S phase, were detected as exceptionally distinct bands. Each late replicating region had its own timing and duration to replicate, which were not influenced by any translocations. This suggested...

Barley allele-specific amplicons useful for identifying wheat-barley recombinant chromosomes.

Barley (Hordeum vulgare L.) is potentially a new source of genes for wheat (Triticum aestivum L.) improvement. Wheat-barley chromosome recombinant lines provide a means for introgressing barley genes to wheat genome by chromosome engineering, and since these are expected to occur only rarely in special cytogenetic stocks, an efficient selection skill is necessary to identify them. To convert RF...